@article{291,
author = "Narasimha Murthy. K and Fazilath Uzma and Soumya. K and Srinivas. C",
abstract = "Bacterial wilt is a major disease in solanaceous crop plants caused by Ralstonia solanacearum. Management of bacterial wilt is very complicated as there are no effective curative chemicals. The present study was conducted to analyze the in vitro antibacterial activity of Azadirachta indica against R.solanacearum. Neem extract was evaluated their antimicrobial activity assayed by three different methods namely, agar dilution, agar well diffusion, and disk inhibition zone methods on Tryptone soya agar (TSA) medium against R. solanacearum. Antibacterial effect of the extracts was studied and compared with commercial antibiotic. The results revealed that the average zone of inhibitions were in the aqueous and methanolic extracts were showed against ten R. solanacearum isolates in the range of 15 mm to 20mm, 13 mm to 21mm and 12mm to 23mm inhibition zone of different three methods respectively mentioned previously. The means and standard error of triplicate tests were recorded. The methanolic extract was found to be more effective than aqueous extract against R. solanacearum. The minimum inhibitory concentration (MIC) was determined by two-fold micro broth dilution method for the tested R. solanacearum. The MIC of the neem methanolic extract and aqueous extracts were 256μg ml-1 and 1024μg ml-1 respectively. Phytochemical analysis of neem leaf extract showed presence of phenols tannins, flavonoids, alkaloids, coumarins, saponins, steroides, terpenes quinones, proteins and amino acids and cardiac glycosides using methanolic extraction of bioactive compounds are solvent dependent. The results revealed that neem extracts have produced inhibitory activity against R. solanacearum and the same could be used to extract antibacterial compounds. The potential of Azadirachta indica extracts was found much better than most of the antibiotics.",
journal = "IJRAS",
keywords = "Bacterial Wilt, Phytochemicals, Neem, Ralstonia solanacearum, Tomato",
month = "September",
note = "This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. CC BY-NC-SA 4.0 Creative Commons License: https://creativecommons.org/licenses/by-nc-sa/4.0/",
number = "5",
pages = "217-223",
title = "{A}ntibacterial {A}ctivity of {N}eem ({A}zadirachta indica) {P}lant {E}xtracts against {B}acterial {W}ilt of {T}omato {C}aused by {R}alstonia solanacearum",
volume = "2",
year = "2015",
}