Effect of Temperature and Duration of Storage on Viability of Plukenetia Conophora (Walnut) Mull Arg Seeds using Tetrazolium Test

Select Volume / Issue:

• Volume 5,Issue 2, Mar. 2018

Year:

2018

Type of Publication:

Article

Keywords:

Viability, Storage Temperature, Tetrazolium Test, Plukenetia Conophora, Seeds, Recalcitrant

Authors:

Amadi, J.O.; Appah, O.R.; Ibode, T.R.; Agbonavbare, O.R.

Journal:

IJRAS

Volume:

5

Number:

2

Pages:

104-107

Month:

March

ISSN:

2348-3997

Note:

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. CC BY-NC-SA 4.0 Creative Commons License: https://creativecommons.org/licenses/by-nc-sa/4.0/

Abstract:

In this study the effect of temperature and duration of storage on viability of Plukenetia conophora seeds using Tetrazolium (T2C) test was investigated. The seeds were subjected to five different storage temperature including a control, viz: room temperature (28°C), freezer (-5°C), refrigerator (7°C), plastic container filled with dry soil (29°C). The seeds were stored in these treatments for six months. For the control, seeds were tested immediately after extraction. Viability test of embryo from 20 seeds per treatment was carried out fortnightly for the six months by soaking in T2C for 24 hours, after which seeds were examined for colour change. Viable seeds changed to red colour. There was significant difference between viability of fresh seeds (100%) and seeds stored at different temperatures at p

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