Antimicrobial Activity of Aqueous Neem Leaves Extracts

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Year:
2024
Type of Publication:
Article
Keywords:
Neem Tree, Aqueous Extracts, Antimicrobial Activity, Gram Positive Bacteria, Gram Negative Bacteria, Yeast, Fungi
Authors:
Jadeja, Gayatree; Shakya, Hitesh
Journal:
IJRAS
Volume:
11
Number:
5
Pages:
71-76
Month:
September
ISSN:
2348-3997
Abstract:
The aqueous extraction of neem leaves were carried out at 60 ˚C under acidic (0.5 N HCL), neutral (D/W) & alkaline (0.5 N NaOH) conditions. Moisture, ash, total protein, soluble protein, carbohydrate and lipid content were determined from fresh neem leaves. The carbohydrate & soluble protein content as well as antimicrobial activities were measured from dry neem leaves. After extraction, the plant material was pretreated with n-Hexane to remove lipids and oils. Soluble protein, carbohydrate as well as antimicrobial effect of aqueous extracts were estimated. All of native extracts at concentration of 20 µ gm/ml had various inhibition activities against the Gram positive bacteria (Staphylococcus aureus), the Gram-negative bacteria (Escherichiacoli, Pseudomonus aeruginos) yeast (Saccharemyce cerevisiaethe) and fungi (Aspergillus niger and Penicillium cryusogenus). In the light of these results we concluded that level of antimicrobial activities of the various extracts depends on both the protein and carbohydrate contents. Generally, the high level of protein and carbohydrate contents of extract had better antimicrobial activities.

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